ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal tRFP tag.
TurboRFP (excitation/ emission max = 553/ 574 nm) is a red (orange) fluorescent protein. TurboRFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboRFP
Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal tRFP-tagging vector, PS100034
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
The use of this Vectors has been cited in the following citations:
Ceramide Synthase 6 Is a Novel Target of Methotrexate Mediating Its Antiproliferative Effect in a p53-Dependent Manner,Fekry, B;Esmaeilniakooshkghazi, A;Krupenko, SA;Krupenko, NI;,PLoS ONE Jan 2016,PubMed ID 26783755[PS100033]
Rab14 regulates the maturation of macrophage phagosomes containing the fungal pathogen Candida albicans and the outcome of the host-pathogen interaction,Okai, B;Lyall, N;Gow, NA;Bain, JM;Erwig, LP;,Infect. Immun. Feb 2015,PubMed ID 25644001[PS100033]
Targeting of aquaporin 4 into lipid rafts and its biological significance,Asakura, K;Ueda, A;Shima, S;Ishikawa, T;Hikichi, C;Hirota, S;Fukui, T;Ito, S;Mutoh, T;,Brain Res. Aug 2014,PubMed ID 25128605[HORT302]