ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal tRFP tag.
TurboRFP (excitation/ emission max = 553/ 574 nm) is a red (orange) fluorescent protein. TurboRFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboRFP
Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal tRFP-tagging vector, PS100034
RFP_F Primer can be used to sequence the target sequence after cloning.
XL39_R Primer can be used to sequence the target sequence after cloning.
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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