pLenti-E/hSyn-C-Myc-DDK-P2A-Puro Lentiviral Gene Expression Vector
pLenti-E/hSyn-C-Myc-DDK-P2A-Puro, Lenti vector with CMV enhancer, Human synapsin I promoter, C-terminal Myc-DDK tag and P2A-Puro, 10 ug
E. coli Selection
This Lenti vector is a third generation lentiviral vector.
The ORF cloned in this vector can be expressed as a tagged fusion protein with C-terminal Myc-DDK tag. The expression of the cloned ORF is driven by CMV enhancer and human synapsin I promoter, a neuron specific promoter. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34ug/ml) All OriGene's TrueORF clone can be shuttled into this vector via
PrecisionShuttling, a simple cut-and-ligation process.
Syn_F Primer can be used to sequence the target sequence after cloning.
P2A-DDK_R Primer can be used to sequence the target sequence after cloning. Download full vector sequence
Schematic of the multiple cloning sites
TurboFectin Transfection Reagent (1 mL in 1 vial)
c-Myc (c-Myc ) mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks