pCas-Guide-EF1a-CD4

CAT#: GE100022

pCas-Guide-EF1a-CD4 vector (with Cas9 and CD4 expression) for genomic target sequence cloning (10 µg)


Reconstitution Protocol

USD 650.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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Specifications

Product Data
Function All-in-One CRISPR/Cas9 vectors
Features

All-in-one vector

  • Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
  • BamH I and BsmB I sites are used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • EF1a promoter driven CD4 to enrich transfected cells
  • CF3 primer (GE100008) can be used to sequence the target sequence after cloning
Disclaimer These products are manufactured and supplied by OriGene under license from ERS.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.