pEX-N-His Bacterial Expression Vector
pEX-N-His, bacterial expression vector with N-terminal His tag, 10ug
TurboFectin Transfection Reagent (1 mL in 1 vial)
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
E. coli Selection
Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal His tag. Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column.
PS100030_F Primer can be used to sequence the target sequence after cloning.
PS100028_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks