pEX-N-GST Bacterial Expression Vector

CAT#: PS100029

pEX-N-GST, bacterial expression vector with N-terminal GST tag, 10ug

Product Images

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Specifications

Product Data

• N-Tag: GST
• E. coli Selection: Ampicillin (100 ug/mL)
• Features:
  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal GST tag.
  • Detection and purification of the transgene are best done using a glutathione affinity column.
  • If the N-terminal tagging interferes with the protein's function, you can choose the C-terminal GST-tagging vector, PS100083
  • PS100028_F Primer can be used to sequence the target sequence after cloning.
  • PS100028_R Primer can be used to sequence the target sequence after cloning.
• Schematic of the multiple cloning sites: