Gch1 Rat siRNA Oligo Duplex (Locus ID 29244)
Gch1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
|Quality Control||Tested by ESI-MS|
|Sequences||Available with shipment|
|Stability||One year from date of shipment when stored at -20°C.|
|Number of Transfections||Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).|
|Note||Single siRNA duplex (10nmol) can be ordered.|
|Components||Gch1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 29244)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml|
|Summary||an enzyme for tetrahydrobiopterin (BH(4)) synthesis; may mediate cell death of striatal neuronal and non-neuronal cells [RGD, Feb 2006]|
|Performance Guranteed||OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).