ROBO1 Human siRNA Oligo Duplex (Locus ID 6091)
ROBO1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
SR321632 is the updated version of SR304090.
|Quality Control||Tested by ESI-MS|
|Sequences||Available with shipment|
|Stability||One year from date of shipment when stored at -20°C.|
|Number of Transfections||Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).|
|Note||Single siRNA duplex (10nmol) can be ordered.|
|RefSeq||NM_001145844, NM_001145845, NM_002941, NM_133631|
|Synonyms||axon guidance receptor; DUTT1; FLJ21882; MGC131599; MGC133277; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout 1; SAX3|
|Components||ROBO1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 6091)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml|
|Summary||Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]|
|Performance Guranteed||OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
|The use of this RNAi has been cited in the following citations:|
Cancer-selective nanoparticles for combinatorial siRNA delivery to primary human GBM in vitro and in vivo
,Kozielski, KL;Ruiz-Valls, A;Tzeng, SY;Guerrero-Cázares, H;Rui, Y;Li, Y;Vaughan, HJ;Gionet-Gonzales, M;Vantucci, C;Kim, J;Schiapparelli, P;Al-Kharboosh, R;Quiñones-Hinojosa, A;Green, JJ;,
,PubMed ID 31026613
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.