DLK1 Human siRNA Oligo Duplex (Locus ID 8788)
DLK1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
|Quality Control||Tested by ESI-MS|
|Sequences||Available with shipment|
|Stability||One year from date of shipment when stored at -20ºC|
|# of transfections||Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM)|
|Note||Single siRNA duplex (10nmol) can be ordered.|
|Synonyms||FA1, ZOG, pG2, PREF1, Pref-1;DLK; FA1; PREF1; Pref-1; ZOG; pG2; delta-like 1 homolog; delta-like homolog; secredeltin; delta-like 1 homolog (Drosophila)|
|Components||DLK1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 8788)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml|
|Summary||May have a role in neuroendocrine differentiation. [UniProtKB/Swiss-Prot Function]|
|Performance Guranteed||OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency.
For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).
|The use of this RNAi has been cited in the following citations:|
The adaptor SASH1 acts through NOTCH1 and its inhibitor DLK1 in a 3D model of lumenogenesis involving CEACAM1.
,Stubblefield, K;Chean, J;Nguyen, T;Chen, CJ;Shively, JE;,
Exp. Cell Res. 2017,PubMed ID 28823832
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