DKK1 Human shRNA Plasmid Kit (Locus ID 22943)

CAT#: TR313465

DKK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

custom shRNA service

USD 695.00


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Size
    • 5 ug/vial

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Specifications

Product Data
Product Name DKK1 Human shRNA Plasmid Kit (Locus ID 22943)
Locus ID 22943
Synonyms DKK-1; SK
Vector pRS
Antibiotic Selection Ampicillin
Format Retroviral plasmids
Kit Components DKK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 22943). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq BC001539, NM_012242
Summary This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations