FIGF Human shRNA Plasmid Kit (Locus ID 2277)

CAT#: TR312980

VEGFD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

custom shRNA service

USD 695.00


Availability*
2 Weeks

Size
    • 5 ug/vial

Product images

Specifications

Product Data
Product Name FIGF Human shRNA Plasmid Kit (Locus ID 2277)
Locus ID 2277
Synonyms VEGF-D; VEGFD
Vector pRS
Antibiotic Selection Ampicillin
Format Retroviral plasmids
Kit Components VEGFD - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 2277). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_004469
Summary The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. [provided by RefSeq, Feb 2011]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.