NANOG Human shRNA Plasmid Kit (Locus ID 79923)
NANOG - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector
|Product Name||NANOG Human shRNA Plasmid Kit (Locus ID 79923)|
|Kit Components||NANOG - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 79923). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.|
|RefSeq||NM_001297698, NM_024865, XM_011520850, XM_011520852|
|Summary||The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
CD133(+) cancer stem cells promoted by VEGF accelerate the recurrence of hepatocellular carcinoma
,Liu, K;Hao, M;Ouyang, Y;Zheng, J;Chen, D;,
Sci Rep 2017,PubMed ID 28134312
Overexpression of NANOG in Gestational Trophoblastic Diseases: Effect on Apoptosis, Cell Invasion, and Clinical Outcome
,Michelle K.Y. Siu, Esther S.Y. Wong, Hoi Yan Chan, Hextan Y.S. Ngan, Kelvin Y.K. Chan, and Annie N.Y. Cheung,
Am. J. Pathol., Oct 2008; 173: 1165 - 1172