MCP1 (CCL2) Human shRNA Plasmid Kit (Locus ID 6347)

CAT#: TL316716

CCL2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL316716A virus into HEK293 cells. TL316716A virus was prepared using lenti-shRNA TL316716A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL316716B virus into HEK293 cells. TL316716B virus was prepared using lenti-shRNA TL316716B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL316716C] virus into HEK293 cells. [TL316716C] virus was prepared using lenti-shRNA [TL316716C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL316716D] virus into HEK293 cells. [TL316716D] virus was prepared using lenti-shRNA [TL316716D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 6347
• Synonyms: GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: CCL2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6347). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_002982, NM_002982.1, NM_002982.2, NM_002982.3, BC009716, BC009716.1, NM_002982.4
• UniProt ID: P13500
• Summary: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. [provided by RefSeq, Aug 2020]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).