CD30 (TNFRSF8) Human shRNA Plasmid Kit (Locus ID 943)

CAT#: TL308730

TNFRSF8 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL308730A virus into HEK293 cells. TL308730A virus was prepared using lenti-shRNA TL308730A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL308730B virus into HEK293 cells. TL308730B virus was prepared using lenti-shRNA TL308730B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL308730C] virus into HEK293 cells. [TL308730C] virus was prepared using lenti-shRNA [TL308730C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL308730D] virus into HEK293 cells. [TL308730D] virus was prepared using lenti-shRNA [TL308730D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 943
• Synonyms: CD30; D1S166E; Ki-1
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: TNFRSF8 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 943). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_001243, NM_001281430, NM_152942, NM_001243.1, NM_001243.2, NM_001243.3, NM_001243.4, NM_152942.1, NM_152942.2, NM_001281430.1, NM_001281430.2, BC063482, BC073839, BC136400, BC141804, BC171762, NM_001243.5
• UniProt ID: P28908
• Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).