E2F1 Human shRNA Plasmid Kit (Locus ID 1869)
E2F1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||E2F1 Human shRNA Plasmid Kit (Locus ID 1869)|
|Synonyms||E2F-1; RBAP1; RBBP3; RBP3|
|Kit Components||E2F1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 1869). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|Summary||The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Cooperative Activation of Tissue-Specific Genes by pRB and E2F1
,Stephen Flowers, Fuhua Xu, and Elizabeth Moran,
Cancer Res., Apr 2013; 73: 2150 - 2158.,PubMed ID 23341573
Transcriptional Regulation of the Human Tumor Suppressor DOK1 by E2F1
,Maha Siouda, Jiping Yue, Ruchi Shukla, Sophie Guillermier, Zdenko Herceg, Marion Creveaux, Rosita Accardi, Massimo Tommasino, and Bakary S. Sylla,
Mol. Cell. Biol., Dec 2012; 32: 4877 - 4890.