LCN2 Human shRNA Plasmid Kit (Locus ID 3934)
LCN2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||LCN2 Human shRNA Plasmid Kit (Locus ID 3934)|
|Synonyms||24p3; MSFI; NGAL|
|Kit Components||LCN2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 3934). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|Summary||This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Lipocalin 2 Enhances Migration and Resistance against Cisplatin in Endometrial Carcinoma Cells
,Miyamoto, T;Kashima, H;Yamada, Y;Kobara, H;Asaka, R;Ando, H;Higuchi, S;Ida, K;Mvunta, DH;Shiozawa, T;,
PLoS ONE May 2016,PubMed ID 27168162
Lipocalin 2 attenuates iron-related oxidative stress and prolongs the survival of ovarian clear cell carcinoma cells by up-regulating the CD44 variant
,Yamada, Y;Miyamoto, T;Kashima, H;Kobara, H;,
Free radical Research Feb 2016,PubMed ID 26729415