COX2 (PTGS2) Human shRNA Plasmid Kit (Locus ID 5743)

CAT#: TG310074

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PTGS2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector



USD 825.00


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Size
    • 5 ug/vial


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Specifications

Product Data
Locus ID 5743
Synonyms COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2
Vector pGFP-V-RS
E. coli Selection Kanamycin
Cell Selection Puromycin
Format Retroviral plasmids
Kit Components PTGS2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 5743). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_000963, NM_000963.1, NM_000963.2, NM_000963.3, BC013734, BC013734.1, BM978796, NM_000963.4
UniProt ID P35354
Summary Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
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