UHRF1 Human shRNA Plasmid Kit (Locus ID 29128)

CAT#: TG308492

UHRF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Specifications

Product Data

• Locus ID: 29128
• Synonyms: hNP95; hUHRF1; huNp95; ICBP90; Np95; RNF106; TDRD22
• Vector: pGFP-V-RS
• E. coli Selection: Kanamycin
• Mammalian Cell Selection: Puromycin
• Format: Retroviral plasmids
• Kit Components: UHRF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 29128). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
• RefSeq: NM_001048201, NM_001290050, NM_001290051, NM_001290052, NM_013282, NM_013282.1, NM_013282.2, NM_013282.3, NM_013282.4, NM_001048201.1, NM_001048201.2, NM_001290050.1, NM_001290051.1, NM_001290052.1, BC113875, BC039136, BC046179, NM_001048201.3, NM_001290051.2
• UniProt ID: Q96T88
• Summary: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).