M-CSF (CSF1) Human shRNA Plasmid Kit (Locus ID 1435)

CAT#: TG305200

CSF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided


USD 883.00

In Stock*

Size
    • 1 kit

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Specifications

Product Data
Locus ID 1435
Synonyms CSF-1; MCSF
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Kit Components CSF1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 1435). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_000757, NM_172210, NM_172211, NM_172212, NM_172212.1, NM_172212.2, NM_172210.1, NM_172210.2, NM_000757.1, NM_000757.2, NM_000757.3, NM_000757.4, NM_000757.5, NM_172211.1, NM_172211.2, NM_172211.3, BC021117, BC021117.1
UniProt ID P09603
Summary The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

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