UBE2I Human shRNA Plasmid Kit (Locus ID 7329)

CAT#: TG300697

UBE2I - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector

custom shRNA service

USD 695.00


Availability*
In Stock

Size
    • 5 ug/vial

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Specifications

Product Data
Product Name UBE2I Human shRNA Plasmid Kit (Locus ID 7329)
Locus ID 7329
Synonyms C358B7.1; P18; UBC9
Vector pGFP-V-RS
Antibiotic Selection Kanamycin
Format Retroviral plasmids
Kit Components UBE2I - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 7329). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq BC000744, NM_003345, NM_194259, NM_194260, NM_194261, XM_005255540, XM_005255541, XM_011522644, XM_011522645, XM_017023640
Summary The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations