WIBG (PYM1) Human shRNA Plasmid Kit (Locus ID 84305)

SKU TG300457

PYM1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Product Data

• Locus ID: 84305
• Synonyms: PYM; WIBG
• Vector: pGFP-V-RS
• E. coli Selection: Kan
• Format: Retroviral plasmids
• Components: PYM1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 84305). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
• RefSeq: NM_001143853, NM_032345, NM_032345.1, NM_032345.2, NM_001143853.1, BC006135, BC006135.1, BC008292, BC009627, BC014976
• UniProt ID: Q9BRP8
• Summary: Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA; the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient