TXK Human shRNA Plasmid Kit (Locus ID 7294)
SKU
TF320564
TXK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
$883.00
In Stock*
Specifications
| Product Data | |
| Locus ID | 7294 |
|---|---|
| Synonyms | BTKL; PSCTK5; PTK4; RLK; TKL |
| Vector | pRFP-C-RS |
| E. coli Selection | Chloramphenicol (34 ug/ml) |
| Format | Retroviral plasmids |
| Components | TXK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 7294). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free. |
| RefSeq | NM_003328, NM_003328.1, NM_003328.2, BC017829, BC166632, NM_003328.3 |
| UniProt ID | P42681 |
| Summary | Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.UniProtKB/Swiss-Prot Function |
| shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
| Shipping | Ambient |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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