EGF Human shRNA Plasmid Kit (Locus ID 1950)

CAT#: TF313278

EGF - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector

custom shRNA service

USD 695.00


Availability*
2 Weeks

Size
    • 5 ug/vial

Product images

Specifications

Product Data
Product Name EGF Human shRNA Plasmid Kit (Locus ID 1950)
Locus ID 1950
Synonyms HOMG4; URG
Vector pRFP-C-RS
Antibiotic Selection Chloramphenicol
Format Retroviral plasmids
Kit Components EGF - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 1950). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001178130, NM_001178131, NM_001963, XM_005262796, XM_011531707, XM_017007845, XM_017007846, XM_017007847, XM_017007848, XM_017007849, XM_017007850, XM_017007851, XM_017007852, XM_017007853, XM_017007854, XM_017007855, XR_001741156, XR_001741157
Summary This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016].
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.