ZDHHC8 Human shRNA Plasmid Kit (Locus ID 29801)
ZDHHC8 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector
|Product Name||ZDHHC8 Human shRNA Plasmid Kit (Locus ID 29801)|
|Synonyms||DHHC8; ZDHHCL1; ZNF378|
|Kit Components||ZDHHC8 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 29801). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.|
|RefSeq||NM_001185024, NM_013373, XM_006724239|
|Summary||This gene encodes a four transmembrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein may function as a palmitoyltransferase. Defects in this gene may be associated with a susceptibility to schizophrenia. Alternate splicing of this gene results in multiple transcript variants. A pseudogene of this gene is found on chromosome 22.[provided by RefSeq, May 2010].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Palmitoylation of cdc42 Promotes Spine Stabilization and Rescues Spine Density Deficit in a Mouse Model of 22q11.2 Deletion Syndrome
,Moutin, E;Nikonenko, I;Stefanelli, T;Wirth, A;Ponimaskin, E;De Roo, M;Muller, D;,
Cereb. Cortex 2016
,PubMed ID 27365300