NANOG Human shRNA Plasmid Kit (Locus ID 79923)

CAT#: TF303039

NANOG - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector

custom shRNA service

USD 695


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Size
    • 5 ug/vial

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Specifications

Product Data
Product Name NANOG Human shRNA Plasmid Kit (Locus ID 79923)
Locus ID 79923
Vector pRFP-C-RS
Antibiotic Selection Chloramphenicol
Format Retroviral plasmids
Kit Components NANOG - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 79923). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq NM_001297698, NM_024865, XM_011520850, XM_011520852
Summary The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations