Furin Mouse shRNA Lentiviral Particle (Locus ID 18550)

CAT#: TL501591V

Furin - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL501591A virus into HEK293 cells. TL501591A virus was prepared using lenti-shRNA TL501591A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL501591B virus into HEK293 cells. TL501591B virus was prepared using lenti-shRNA TL501591B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL501591C] virus into HEK293 cells. [TL501591C] virus was prepared using lenti-shRNA [TL501591C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL501591D] virus into HEK293 cells. [TL501591D] virus was prepared using lenti-shRNA [TL501591D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 18550
• Synonyms: 9130404I01Rik; Fu; Fur; PA; PACE; Pcs; Pcsk3; SP; SPC1
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: BC048234, NM_001081454, NM_011046, NM_001081454.1, NM_001081454.2, NM_011046.1, NM_011046.2, NM_011046.3, BC006686
• UniProt ID: P23188
• Summary: This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).