bcl 6 (BCL6) Human shRNA Lentiviral Particle (Locus ID 604)

CAT#: TL306420V

BCL6 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL306420A virus into HEK293 cells. TL306420A virus was prepared using lenti-shRNA TL306420A and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of TL306420B virus into HEK293 cells. TL306420B virus was prepared using lenti-shRNA TL306420B and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL306420C] virus into HEK293 cells. [TL306420C] virus was prepared using lenti-shRNA [TL306420C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL306420D] virus into HEK293 cells. [TL306420D] virus was prepared using lenti-shRNA [TL306420D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 604
• Synonyms: BCL5; BCL6A; LAZ3; ZBTB27; ZNF51
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001130845, NM_001134738, NM_001706, NM_138931, NM_001706.1, NM_001706.2, NM_001706.3, NM_001706.4, NM_001134738.1, NM_001130845.1, NM_138931.1, BC142705, BC146796, BC150184, NM_001706.5, NM_001130845.2
• UniProt ID: P41182
• Summary: The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).