Dead Cas9 Streptococcus Pyogenes Recombinant Protein

CAT#: TP790184

Purified recombinant protein of mutant(D10A/H840A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- terminal of the protein, with N-terminal HIS tag, expressed in E. coli

Product Images

Purified recombinant protein Dead Cas9 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Streptococcus Pyogenes
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMDKKYS IGLAIGTNSV
  GWAVITDEYK VPSKKFKVLG NTDRHSIKKN LIGALLFDSG ETAEATRLKR
  TARRRYTRRK NRICYLQEIF SNEMAKVDDS FFHRLEESFL VEEDKKHERH
  PIFGNIVDEV AYHEKYPTIY HLRKKLVDST DKADLRLIYL ALAHMIKFRG
  HFLIEGDLNP DNSDVDKLFI QLVQTYNQLF EENPINASGV DAKAILSARL
  SKSRRLENLI AQLPGEKKNG LFGNLIALSL GLTPNFKSNF DLAEDAKLQL
  SKDTYDDDLD NLLAQIGDQY ADLFLAAKNL SDAILLSDIL RVNTEITKAP
  LSASMIKRYD EHHQDLTLLK ALVRQQLPEK YKEIFFDQSK NGYAGYIDGG
  ASQEEFYKFI KPILEKMDGT EELLVKLNRE DLLRKQRTFD NGSIPHQIHL
  GELHAILRRQ EDFYPFLKDN REKIEKILTF RIPYYVGPLA RGNSRFAWMT
  RKSEETITPW NFEEVVDKGA SAQSFIERMT NFDKNLPNEK VLPKHSLLYE
  YFTVYNELTK VKYVTEGMRK PAFLSGEQKK AIVDLLFKTN RKVTVKQLKE
  DYFKKIECFD SVEISGVEDR FNASLGTYHD LLKIIKDKDF LDNEENEDIL
  EDIVLTLTLF EDREMIEERL KTYAHLFDDK VMKQLKRRRY TGWGRLSRKL
  INGIRDKQSG KTILDFLKSD GFANRNFMQL IHDDSLTFKE DIQKAQVSGQ
  GDSLHEHIAN LAGSPAIKKG ILQTVKVVDE LVKVMGRHKP ENIVIEMARE
  NQTTQKGQKN SRERMKRIEE GIKELGSQIL KEHPVENTQL QNEKLYLYYL
  QNGRDMYVDQ ELDINRLSDY DVDAIVPQSF LKDDSIDNKV LTRSDKNRGK
  SDNVPSEEVV KKMKNYWRQL LNAKLITQRK FDNLTKAERG GLSELDKAGF
  IKRQLVETRQ ITKHVAQILD SRMNTKYDEN DKLIREVKVI TLKSKLVSDF
  RKDFQFYKVR EINNYHHAHD AYLNAVVGTA LIKKYPKLES EFVYGDYKVY
  DVRKMIAKSE QEIGKATAKY FFYSNIMNFF KTEITLANGE IRKRPLIETN
  GETGEIVWDK GRDFATVRKV LSMPQVNIVK KTEVQTGGFS KESILPKRNS
  DKLIARKKDW DPKKYGGFDS PTVAYSVLVV AKVEKGKSKK LKSVKELLGI
  TIMERSSFEK NPIDFLEAKG YKEVKKDLII KLPKYSLFEL ENGRKRMLAS
  AGELQKGNEL ALPSKYVNFL YLASHYEKLK GSPEDNEQKQ LFVEQHKHYL
  DEIIEQISEF SKRVILADAN LDKVLSAYNK HRDKPIREQA ENIIHLFTLT
  NLGAPAAFKY FDTTIDRKRY TSTKEVLDAT LIHQSITGLY ETRIDLSQLG
  GDPKKKRKVE DPKKKRKVDT R
• Tag: N-His
• Predicted MW: 164.5 kDa
• Concentration: 20uM
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
• Endotoxin: < 0.5 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20℃.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.