LbCpf1 Lachnospiraceae bacterium ND2006 Recombinant Protein

CAT#: TP790181

Purified recombinant protein of Lachnospiraceae bacterium ND2006 LbCpf1 containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-terminal of the protein, with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture

Product Images

Purified recombinant protein LbCpf1 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Lachnospiraceae bacterium ND2006
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  1 MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMSKLEK FTNCYSLSKT
  51 LRFKAIPVGK TQENIDNKRL LVEDEKRAED YKGVKKLLDR YYLSFINDVL
  101 HSIKLKNLNN YISLFRKKTR TEKENKELEN LEINLRKEIA KAFKGNEGYK
  151 SLFKKDIIET ILPEFLDDKD EIALVNSFNG FTTAFTGFFD NRENMFSEEA
  201 KSTSIAFRCI NENLTRYISN MDIFEKVDAI FDKHEVQEIK EKILNSDYDV
  251 EDFFEGEFFN FVLTQEGIDV YNAIIGGFVT ESGEKIKGLN EYINLYNQKT
  301 KQKLPKFKPL YKQVLSDRES LSFYGEGYTS DEEVLEVFRN TLNKNSEIFS
  351 SIKKLEKLFK NFDEYSSAGI FVKNGPAIST ISKDIFGEWN VIRDKWNAEY
  401 DDIHLKKKAV VTEKYEDDRR KSFKKIGSFS LEQLQEYADA DLSVVEKLKE
  451 IIIQKVDEIY KVYGSSEKLF DADFVLEKSL KKNDAVVAIM KDLLDSVKSF
  501 ENYIKAFFGE GKETNRDESF YGDFVLAYDI LLKVDHIYDA IRNYVTQKPY
  551 SKDKFKLYFQ NPQFMGGWDK DKETDYRATI LRYGSKYYLA IMDKKYAKCL
  601 QKIDKDDVNG NYEKINYKLL PGPNKMLPKV FFSKKWMAYY NPSEDIQKIY
  651 KNGTFKKGDM FNLNDCHKLI DFFKDSISRY PKWSNAYDFN FSETEKYKDI
  701 AGFYREVEEQ GYKVSFESAS KKEVDKLVEE GKLYMFQIYN KDFSDKSHGT
  751 PNLHTMYFKL LFDENNHGQI RLSGGAELFM RRASLKKEEL VVHPANSPIA
  801 NKNPDNPKKT TTLSYDVYKD KRFSEDQYEL HIPIAINKCP KNIFKINTEV
  851 RVLLKHDDNP YVIGIDRGER NLLYIVVVDG KGNIVEQYSL NEIINNFNGI
  901 RIKTDYHSLL DKKEKERFEA RQNWTSIENI KELKAGYISQ VVHKICELVE
  951 KYDAVIALED LNSGFKNSRV KVEKQVYQKF EKMLIDKLNY MVDKKSNPCA
  1001 TGGALKGYQI TNKFESFKSM STQNGFIFYI PAWLTSKIDP STGFVNLLKT
  1051 KYTSIADSKK FISSFDRIMY VPEEDLFEFA LDYKNFSRTD ADYIKKWKLY
  1101 SYGNRIRIFR NPKKNNVFDW EEVCLTSAYK ELFNKYGINY QQGDIRALLC
  1151 EQSDKAFYSS FMALMSLMLQ MRNSITGRTD VDFLISPVKN SDGIFYDSRN
  1201 YEAQENAILP KNADANGAYN IARKVLWAIG QFKKAEDEKL DKVKIAISNK
  1251 EWLEYAQTSV KHPKKKRKVE DPKKKRKVDT R
• Tag: N-His
• Predicted MW: 149.8 kDa
• Concentration: 5uM
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
• Endotoxin: < 0.5 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20℃ after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.