Cardiac Troponin I (TNNI3) (NM_000363) Human Recombinant Protein

CAT#: TP750154

Purified recombinant protein of Human troponin I type 3 (cardiac) (cTnI), full length, with N-terminal His tag, expressed in E.coli, 100ug

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length TNNI3
• Tag: N-His
• Predicted MW: 23.8 kDa
• Concentration: Concentration is determined by spectrophotometrically（A280）
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from 0.01 M HCl, reconstitute with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol)
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -20°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000354
• Locus ID: 7137
• UniProt ID: P19429, Q6FGX2
• Cytogenetics: 19q13.42
• Refseq Size: 2073
• Refseq ORF: 630
• Synonyms: CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1
• Summary: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19. [provided by RefSeq, Aug 2020]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)