H3FT (HIST3H3) (NM_003493) Human Recombinant Protein

CAT#: TP750003

Recombinant protein of human Histone H3 (HIST3H3) produced in E. coli.

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Met1-Ala13polyhistidinetone H3
• Tag: Tag Free
• Predicted MW: 15.5 kDa
• Concentration: Resuspend the protein to the desired concentration in proper buffer.
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a sterile solution containing 20 mM PB, pH 7.4
• Endotoxin: < 0.1 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_003484
• Locus ID: 8290
• UniProt ID: Q16695
• Cytogenetics: 1q42.13
• Refseq Size: 481
• Refseq ORF: 408
• Synonyms: H3.4; H3/g; H3FT; H3t; HIST3H3
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
• Protein Pathways: Systemic lupus erythematosus