IL17 (IL17A) (NM_002190) Human Recombinant Protein

CAT#: TP723780

Purified recombinant protein of Human interleukin 17A and 17F (IL17A / IL-17F) heterodimer

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human IL-17A, the region of Ile20-Ala155, from gene Accession# NM_002190 and human IL-17F, the region of Arg31-Gln163, from gene Accession# AF384857
• Tag: Tag Free
• Predicted MW: 30.6 kDa
• Concentration: lot specific
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10 mM NaH2PO4, 300 mM NaCl, pH 7.2
• Bioactivity: The ED50 is 15-25 ng/ml, corresponding to a specific activity of 6.6 - 4 x 10^4 units/mg. Under this assay the activity was comparable to other vendor's cytokine.
• Endotoxin: Less than 0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_002181
• Locus ID: 3605
• UniProt ID: Q16552
• Cytogenetics: 6p12.2
• Refseq Size: 1859
• Refseq ORF: 465
• Synonyms: CTLA-8; CTLA8; IL-17; IL-17A; IL17; ILA17
• Summary: This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A. [provided by RefSeq, Sep 2020]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction