Thpo (NM_031133) Rat Recombinant Protein
Purified recombinant protein of Rat thrombopoietin (Thpo).
|Description||Purified recombinant protein of Rat thrombopoietin (Thpo).|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||SPVPPACDPRLLNKLLRDSYLLHSRLSQCPDVNPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPPQGRTTAHKDPSALFLSLQQLLRGKVRFLLLVEGPALCVRRTLPTTAVPSRTSQLLTLNKF|
|Predicted MW||18.7 kDa|
|Purity||>95% as determined by SDS-PAGE and Coomassie blue staining|
|Buffer||Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2|
|Bioactivity||Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is less than or equal to 0.2 ng/ml, corresponding to a specific activity of > 5 x 10^6 units/mg.|
|Endotoxin||Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)|
|Storage||Store at -80°C.|
|Stability||Stable for at least 6 months from date of receipt under proper storage and handling conditions.|
|Summary||lineage-specific cytokine; important for proliferation and development of megakaryocytes and platelets; may be the major regulator of circulating platelets [RGD, Feb 2006]|
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