Lep (NM_013076) Rat Recombinant Protein
Purified recombinant protein of Rat leptin (Lep).
|Description||Purified recombinant protein of Rat leptin (Lep).|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MVPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC|
|Predicted MW||16.2 kDa|
|Purity||>95% as determined by SDS-PAGE and Coomassie blue staining|
|Buffer||Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2|
|Bioactivity||Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5µg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.|
|Endotoxin||Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)|
|Storage||Store at -80°C.|
|Stability||Stable for at least 6 months from date of receipt under proper storage and handling conditions.|
|Summary||involved in regulation of body weight, and may have a role in angiogenesis [RGD, Feb 2006]|
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