Esyt1 (NM_011843) Mouse Recombinant Protein

CAT#: TP511619

Purified recombinant protein of Mouse extended synaptotagmin-like protein 1 (Esyt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >MR211619 representing NM_011843
  Red=Cloning site Green=Tags(s)
  
  MEHSPEEGASPEPSGQPPATDSTRDGGSGVPPAGPGAASEALAVLTSFGRRLLVLVPVYLAGAAGLSVGF
  VLFGLALYLGWRRVRDGKERSLRAARQLLDDEERITAETLYMSHRELPAWVSFPDVEKAEWLNKIVAQVW
  PFLGQYMEKLLAETVAPAVRGANPHLQTFTFTRVELGEKPLRIIGVKVHPSQRKDQILLDLNVSYVGDVQ
  IDVEVKKYFCKAGVKGMQLHGVLRVILEPLTGDLPIVGAVSMFFIKRPTLDINWTGMTNLLDIPGLSSLS
  DTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYA
  LVRVGTQTFCSRVIDEELNPHWGETYEVIVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDVGKVLQAGVLD
  NWYPLQGGQGQVHLRLEWLSLLPDAEKLDQVLQWNRGITSRPEPPSAAILVVYLDRAQDLPLKKGNKEPN
  PMVQLSVQDVTRESKATYSTNSPVWEEAFRFFLQDPRSQELDVQVKDDSRALTLGALTLPLARLLTASEL
  TLDQWFQLSSSGPNSRLYMKLVMRILYLDYSEIRFPTVPGAQDWDRESLETGSSVDAPPRPYHTTPNSHF
  GTENVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKVAGKSFRTHVVREDLNPRWNEVFEVIVTSIPG
  QELEIEVFDKDLDKDDFLGRYKVSLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRPTAAELEEVLQV
  NSLIQTQKSSELAAALLSVFLERAEDLPLRKGTKPPSPYATITVGETSHKTKTVSQSSAPVWEESASFLI
  RKPHAESLELQVRGEGTGTLGSVSLPLSELLQEDQLCLDHWFALSGQGQVLMRAQLGILVSQHSGVEAHS
  HSYSHSHSSSSLNDEPEALGGPTHPASPVLEVRHRLTHGDSPSEAPVGPLGQVKLTVWYHSDEQKLISII
  HSCRALRQNGRDLPDPYVSVLLLPDKNRSTKRKTPQKKRTLNPEFNERFEWDLPLDGTLRRKLDVSVKSN
  SSFMSRERELLGKVQLDLAEIDLSQGAAQWYDLMDDRDKGGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-MYC/DDK
• Predicted MW: 122 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_035973
• Locus ID: 23943
• UniProt ID: Q3U7R1
• Cytogenetics: 10 D3
• Refseq Size: 3687
• Refseq ORF: 3276
• Synonyms: Fam62a; Mbc2; vp115
• Summary: Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]