Nucleoside phosphorylase (PNP) (NM_000270) Human Recombinant Protein
Specifications
Product Data | |
Description | Recombinant protein of human nucleoside phosphorylase (NP) |
Species | Human |
Expression Host | HEK293T |
Tag | C-Myc/DDK |
Predicted MW | 31.9 kDa |
Concentration | >50 ug/mL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol |
Bioactivity | WB standard (PMID: 28251649) |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Reference Data | |
RefSeq | NP_000261 |
Locus ID | 4860 |
Refseq Size | 2438 |
Cytogenetics | 14q11.2 |
Refseq ORF | 867 |
Synonyms | NP; PRO1837; PUNP |
Summary | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008] |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Evidence for purine nucleoside phosphorylase (PNP) release from RAT C6 glioma cells
,Giuliani, P;Zuccarini, M;Buccella, S;Peña-Altamira, LE;Polazzi, E;Virgili, M;Monti, B;Poli, A;Rathbone, MP;Di Iorio, P;Ciccarelli, R;Caciagli, F;,
J. Neurochem 2017
,PubMed ID 28251649
[PNP]
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Documents
FAQs |
SDS |
Resources
Protein Resources |
Other Versions
SKU | Description | Size | Price |
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LY424829 | Transient overexpression lysate of purine nucleoside phosphorylase (PNP) |
USD 315.00 |
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PH310759 | PNP MS Standard C13 and N15-labeled recombinant protein (NP_000261) |
USD 1,995.00 |
|
AR09725PU-L | Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
USD 820.00 |
|
AR09725PU-N | Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
USD 320.00 |
|
LC424829 | PNP HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
USD 299.00
USD 360.00