TSSK1(TSSK1B) (NM_032028) Human Recombinant Protein

CAT#: TP305395

Recombinant protein of human testis-specific serine kinase 1B (TSSK1B)

Bulk Request/Custom Protein Development

 Product Datasheet for 'TP305395'

USD 680.00

In Stock

    • 20 ug

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Product Data
Description Recombinant protein of human testis-specific serine kinase 1B (TSSK1B)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC205395. Click on the TrueORF clone link to view cDNA and protein sequences.
Predicted MW 41.4 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity TSSK1B activity verified in a biochemical assay: TSSK1B (testis-specific serine kinase 1B) (TP305395) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. TSSK1B is a serine/threonine kinase that is highly expressed in testis and may be involved in a signaling pathway during male germ cell development or mature sperm function. Varying concentrations of TSSK1B were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Reference Data
RefSeq NP_114417
Locus ID 83942
Refseq Size 2012
Cytogenetics 5q22.2
Refseq ORF 1101
Synonyms FKSG81; SPOGA4; STK22D; TSK1; TSSK1
Summary TSSK1 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM, Mar 2008].
Protein Families Protein Kinase, Druggable Genome
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