RDH11 (NM_016026) Human Recombinant Protein

CAT#: TP305003M

Recombinant protein of human retinol dehydrogenase 11 (all-trans/9-cis/11-cis) (RDH11), 100 µg

Product Images

The expression levels of microsomal RDH11 in four human livers are shown using either recombinant protein standard (OriGene TP305003) or the AQUA method as calibrators in LC-MS/MS assays. Figure cited from Anal Chem, PMID: 26606514

• 

  Coomassie blue staining of purified RDH11 protein (Cat# TP305003). The protein was produced from HEK293T cells transfected with RDH11 cDNA clone (Cat# RC205003) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC205003 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVELMFPLLLLLLPFLLYMAAPQIRKMLSSGVCTSTVQLPGKVVVVTGANTGIGKETAKELAQRGARVYL
  ACRDVEKGELVAKEIQTTTGNQQVLVRKLDLSDTKSIRAFAKGFLAEEKHLHVLINNAGVMMCPYSKTAD
  GFEMHIGVNHLGHFLLTHLLLEKLKESAPSRIVNVSSLAHHLGRIHFHNLQGEKFYNAGLAYCHSKLANI
  LFTQELARRLKGSGVTTYSVHPGTVQSELVRHSSFMRWMWWLFSFFIKTPQQGAQTSLHCALTEGLEILS
  GNHFSDCHVAWVSAQARNETIARRLWDVSCDLLGLSID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 35.2 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Bioactivity: MS digestion standard (PMID: 26606514)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_057110
• Locus ID: 51109
• UniProt ID: Q8TC12, A0A0S2Z583
• Cytogenetics: 14q24.1
• Refseq Size: 2587
• Refseq ORF: 954
• Synonyms: ARSDR1; CGI82; HCBP12; MDT1; PSDR1; RALR1; RDJCSS; SCALD; SDR7C1
• Summary: The protein encoded by this gene is an NADPH-dependent retinal reductase and a short-chain dehydrogenase/reductase. The encoded protein has no steroid dehydrogenase activity. [provided by RefSeq, Nov 2011]
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism