GGH (NM_003878) Human Recombinant Protein

CAT#: TP302265

Recombinant protein of human gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) (GGH), 20 µg

Product Images

Coomassie blue staining of purified GGH protein (Cat# TP302265). The protein was produced from HEK293T cells transfected with GGH cDNA clone (Cat# RC202265) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC202265 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPGCLLCVLGLLLCGAASLELSRPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGAR
  VVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEEL
  SLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKL
  KKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEAR
  KNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 33.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_003869
• Locus ID: 8836
• UniProt ID: Q92820
• Cytogenetics: 8q12.3
• Refseq Size: 1505
• Refseq ORF: 954
• Synonyms: GATD10; GH
• Summary: This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Folate biosynthesis