PAM (NM_138821) Human Recombinant Protein

CAT#: TP302074

Recombinant protein of human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3

Bulk Request/Custom Protein Development

 Product Datasheet for 'TP302074'

USD 438.00

USD 730.00

In Stock

    • 20 ug

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Product Data
Description Recombinant protein of human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC202074. Click on the TrueORF clone link to view cDNA and protein sequences.
Predicted MW 94.2 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity The specific activity of PAM was determined by measuring the product D-Tyr-Val-NH2 formation from a conversion of D-Tyr-Val-Gly. The reaction was carried out at 37C for 60min in the buffer containing 50 mM MES, pH6.0, 1 µM CuCl2, 2000 units/ml of Catalase, 5 mM L-ascorbic acid, and 50 uM of D-Tyr-Val-Gly as the substrate
Reference Data
RefSeq NP_620176
Locus ID 5066
Refseq Size 3636
Cytogenetics 5q21.1
Refseq ORF 2598
Synonyms PAL; PHM
Summary Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProtKB/Swiss-Prot Function]
Protein Families Transmembrane, Druggable Genome
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