SF3B5 (NM_031287) Human Recombinant Protein

CAT#: TP300977L

Recombinant protein of human splicing factor 3b, subunit 5, 10kDa (SF3B5), 1 mg

Product Images

Coomassie blue staining of purified SF3B5 protein (Cat# TP300977). The protein was produced from HEK293T cells transfected with SF3B5 cDNA clone (Cat# RC200977) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC200977 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLME
  KMLQPCGPPADKPEEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 10 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_112577
• Locus ID: 83443
• UniProt ID: Q9BWJ5
• Cytogenetics: 6q24.2
• Refseq Size: 786
• Refseq ORF: 258
• Synonyms: SF3b10; Ysf3
• Summary: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).[UniProtKB/Swiss-Prot Function]
• Protein Pathways: Spliceosome