TFIIF (GTF2F2) (NM_004128) Human Recombinant Protein

CAT#: TP300361

Recombinant protein of human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2), 20 µg

Product Images

Coomassie blue staining of purified GTF2F2 protein (Cat# TP300361). The protein was produced from HEK293T cells transfected with GTF2F2 cDNA clone (Cat# RC200361) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC200361 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGG
  KPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVR
  LSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPV
  VYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 28.2 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_004119
• Locus ID: 2963
• UniProt ID: P13984, A0A024RDU9
• Cytogenetics: 13q14.12-q14.13
• Refseq Size: 1469
• Refseq ORF: 747
• Synonyms: BTF4; RAP30; TF2F2; TFIIF
• Summary: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.[UniProtKB/Swiss-Prot Function]
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors