MAPKAP Kinase 3(MAPKAPK3) (NM_004635) Human Recombinant Protein

CAT#: TP300358

Recombinant protein of human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)

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 Product Datasheet for 'TP300358'

USD 438.00

USD 730.00


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Size
    • 20 ug

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Specifications

Product Data
Description Recombinant protein of human mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3)
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC200358. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 42.8 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bioactivity MAPKAPK3 activity verified in a biochemical assay: MAPKAPK3 (mitogen-activated protein kinase-activated protein kinase 3) (TP300358) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MAPKAPK3 is a serine/threonine kinase that functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. Varying concentrations of MAPKAPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Reference Data
RefSeq NP_004626
Locus ID 7867
Refseq Size 2500
Cytogenetics 3p21.2
Refseq ORF 1146
Synonyms 3PK; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MK-3
Summary This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Protein Families
Protein Pathways
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