Tumor necrosis factor (TNF-alpha) (77-233)

CAT#: SA6055X

The active form of this protein is a trimer. Recombinant human TNF-a was expressed in E. coli. Additional amino acid, Methionine, was attached at N-terminus of the protein.


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    • 0.5 mg


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Specifications

Product Data
Description The active form of this protein is a trimer. Recombinant human TNF-a was expressed in E. coli. Additional amino acid, Methionine, was attached at N-terminus of the protein.
Species Human
Expression Host E.Coli
Expression cDNA Clone or AA Sequence MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Tag Tag Free
Predicted MW 17.5 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS-PAGE
Buffer PBS, pH 7.4
Bioactivity Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect
is < 0.16 ng/ml.
Endotoxin < 1.0 EU per 1 µg of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000585
Locus ID 7124
Cytogenetics 6p21.33
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Protein Families Secreted Protein, Transmembrane, Transcription Factors, Druggable Genome
Protein Pathways MAPK signaling pathway, Cytokine-cytokine receptor interaction, Apoptosis, TGF-beta signaling pathway, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Hematopoietic cell lineage, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Fc epsilon RI signaling pathway, Adipocytokine signaling pathway, Type II diabetes mellitus, Type I diabetes mellitus, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Asthma, Systemic lupus erythematosus, Allograft rejection, Graft-versus-host disease, Hypertrophic cardiomyopathy (HCM), Dilated cardiomyopathy

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