Tumor necrosis factor (TNF-alpha) (77-233)

CAT#: SA6055

Reviews ()
Write a review

The active form of this protein is a trimer. Recombinant human TNF-a was expressed in E. coli. Additional amino acid, Methionine, was attached at N-terminus of the protein.


 Product Datasheet for 'SA6055'

  View other "TNF" proteins (14)

USD 250.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Description The active form of this protein is a trimer. Recombinant human TNF-a was expressed in E. coli. Additional amino acid, Methionine, was attached at N-terminus of the protein.
Species Human
Expression Host E. coli
Predicted MW 17.5 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS-PAGE
Buffer PBS, pH 7.4
Bioactivity Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect
is < 0.16 ng/ml.
Endotoxin < 1.0 EU per 1 µg of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000585
Locus ID 7124
Cytogenetics 6p21.33
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

Documents

Other Versions

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones