Protein A (FITC conjugated) Staphylococcus aureus Protein

CAT#: RA100F

Protein A (FITC conjugated) s. aureus protein, 5 mg

Specifications

Product Data

• Species: Staphylococcus aureus
• Concentration: 1.0 mg/ml after reconstitution
• Purity: This product was prepared from chromatographically pure Protein A.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Protein A.
• Conjugation: FITC
• Buffer: State: Lyophilized purified Ig fraction.
  Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons).
  Absorption/Emission Wavelength: 495 nm/528 nm.
  Fluorochrome/Protein Ratio: 4.6 moles FITC per mole of Protein A.
  Presentation Label: FITC
• Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).
• Preparation: Lyophilized purified Ig fraction.
• Applications: Immunofluorescence Microscopy (1/1,000-1/5,000)
  Flow Cytometry/FACS (1/1,000-1/5,000) as well as other antibody based fluorescent assays.
• Protein Description: Fluorescein Conjugated Protein A.
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8°C as undiluted liquid. Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence.
  Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.