Collagen type IV Human Protein

CAT#: RA007

Collagen type IV human protein, 0.5 mg

Specifications

Product Data

• Species: Human
• Protein Source: Placenta
• Concentration: lot specific
• Purity: Chromatographically and Immunologically pure.
• Buffer: State: Liquid (sterile filtered) purified protein
  Buffer System: 0.5M Acetic Acid
  Preservative: 0.01% Sodium Azide
  Stabilizer: None
• Preparation: Liquid (sterile filtered) purified protein
• Applications: ELISA.
  Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
  Immunoprecipitation.
  Western blotting.
• Protein Description: This antigen is free from other Collagens, Human serum proteins and non-Collagen extracellular matrix proteins and reacts with anti-Collagen Type IV.
  Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
• Note: Caution: No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDCNIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same.
  Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Storage: Store vial at 2-8°C prior to opening.
  This product is stable 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
• Stability: Shelf life: 6 months from despatch.

Reference Data

• RefSeq: NP_001290039
• Locus ID: 1282
• Cytogenetics: 13q34
• Synonyms: BSVD; BSVD1; COL4A1s; PADMAL; RATOR
• Summary: This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer