IFN-alpha A/D Bgl II (Universal Type I Interferon) Human Protein

CAT#: PBL11200-2

IFN-alpha A/D Bgl II (Universal Type I Interferon) human recombinant protein, 1 x 10^6 U


Size
    • 1 x 10⁶ U

Product Images

Specifications

Product Data
Species Human
Expression Host E. coli
Predicted MW 19.4
Purity >95% Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel permeation chromatography
Buffer Buffer System: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Bioactivity Biological: Assay Used to Measure Bioactivity: Interferon was titrated in the Cytopathic Effect Inhibition (CPE) assays as listed.
Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml.
Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml. 
Mouse (L929/EMCV) – developed and performed at PBL Biomedical Laboratories. The EC50 for interferon in this assay is ~5 U/ml.

Specific: x 10 units/mg (MDBK/VSV)
Endotoxin < 1 EU/μg
Protein Description Recombinant Human Interferon Alpha hybrid protein; Universal Type I IFN is an alpha interferon hybrid (Human Interferon Alpha A/D [BglII]) constructed from recombinant Human IFN alpha A and Human IFN alpha D. Activity has been observed on many mammalian cells, including human, monkey, mouse, bovine, rat, cat, pig, rabbit, guinea pig, or hamster. This product is useful for cross-species testing with Interferon or for species where autologous interferon is not available.
Note The units are determined by use of a Human IFN Alpha A (Hu-IFN-αA [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu-IFN-αA [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol. Methods. 257(1-2):17-33]. On L929 cells the laboratory standard has been calibrated to the reference standard for Mouse Interferon Alpha provided by the National Institutes of Health. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.