IFN-alpha A/D Bgl II (Universal Type I Interferon) Human Protein
CAT#: PBL11200-2
IFN-alpha A/D Bgl II (Universal Type I Interferon) human recombinant protein, 1 x 10^6 U
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Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Predicted MW | 19.4 |
Purity | >95% Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel permeation chromatography |
Buffer | Buffer System: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
Bioactivity | Biological: Assay Used to Measure Bioactivity: Interferon was titrated in the Cytopathic Effect Inhibition (CPE) assays as listed. Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml. Mouse (L929/EMCV) – developed and performed at PBL Biomedical Laboratories. The EC50 for interferon in this assay is ~5 U/ml. Specific: x 10 units/mg (MDBK/VSV) |
Endotoxin | < 1 EU/μg |
Protein Description | Recombinant Human Interferon Alpha hybrid protein; Universal Type I IFN is an alpha interferon hybrid (Human Interferon Alpha A/D [BglII]) constructed from recombinant Human IFN alpha A and Human IFN alpha D. Activity has been observed on many mammalian cells, including human, monkey, mouse, bovine, rat, cat, pig, rabbit, guinea pig, or hamster. This product is useful for cross-species testing with Interferon or for species where autologous interferon is not available. |
Note | The units are determined by use of a Human IFN Alpha A (Hu-IFN-αA [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu-IFN-αA [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol. Methods. 257(1-2):17-33]. On L929 cells the laboratory standard has been calibrated to the reference standard for Mouse Interferon Alpha provided by the National Institutes of Health. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1]. |
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