Adiponectin (biologically active) Human Protein

CAT#: PA500X

Adiponectin (biologically active) human protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK
  Glu 1 to Gln 5 were confirmed by N-terminal sequencing.
• Concentration: lot specific
• Purity: >98%
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified Protein
  Buffer System: 20 mM Tris buffer, 20 mM NaCl, pH 7.5
• Bioactivity: Biological: In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the human adiponectin derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.
• Reconstitution: Restore with deionized water
• Preparation: Lyophilized purified Protein
• Applications: Cell culture and/or animal studies.
  ELISA.
  Western Blot.
• Protein Description: Human Adiponectin
  Total 226 AA, UniProt Q15848 (Glu19 – Asn244)
• Note: Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
  Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001171271
• Locus ID: 9370
• UniProt ID: Q15848, A8K660, B2R773
• Cytogenetics: 3q27.3
• Synonyms: ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
• Summary: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus