FGF acidic / FGF1 (Cell Culture Grade)

CAT#: DA3556X

Porcine Endothelial Cell Growth Factor (ECGF) (Cell Culture Grade).

Specifications

Product Data

• Description: Porcine Endothelial Cell Growth Factor (ECGF) (Cell Culture Grade).
• Species: Human
• Expression Host: Brain
• Buffer: H2O without preservatives
• Bioactivity: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 µg/ml, optimal concentration with heparin (50 µg/ml) is about 12 µg/ml.
• Reconstitution: Restore the contents of the vial in 2 ml of prewarmed (37°C) sterile balanced salt solution.
  Gently rotate the vial until the contents are dissolved. This stock solution may be further diluted in sterile tissue culture media to obtain the desired working concentrations. Although the stock solution can be added aseptically to sterile tissue culture medium, it is recommended that medium containing diluted product is aseptically filtered prior to use.
  6 mg of ECGF are sufficient for 500 ml medium.
• Preparation: Lyophilized (Sterile Freeze dried) Crude extract

Reference Data

• RefSeq: NP_000791
• Locus ID: 2246
• Cytogenetics: 5q31.3
• Synonyms: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1
• Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton